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Experimental results obtained from 2D cultures under flow conditions
Culturing cells on bi-dimensional substrates is the most simple technique in Tissue Engineering, since many of the shortcomings associated to the lack of vascularisation are absent.
Thus, bi-dimensional cultures are perfect candidates to study the influence of a huge amount of factors thank to its simplicity. Among them, the study of mechanical stimuli as, e.g., shear stress due to culture media flow is one of the most important.
This sort of mechanical stimuli has been experimentally shown to exert a very important influence on certain types of cells, causing the upregulation of a variety of cellular processes.
In order to check the influence of such stimuli, an experimental set-up using the TEB1000 Master Unit was carried out.
Materials and methods
The aim of this experiment was to check the influence of shear stress mechanical stimuli on cells populations due to culture media flow. For such purpose, the following materials were used:
Bioreactor: TEB1000 Master Unit was used to develop the cultures.
Cell type: hBMMSCs.
Culture chambers: Commercial devices Ibidi were used to seed cells subjected to medium flow.
Culture time: 7 days.
Simultaneous experiments: up to twelve.
With these materials, several cultures at different flow rates were developed in order to observe its influence on cell development.
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Results
Cell number vs. flow rate
The most relevant results of the experiments are depicted in the figure beside, where the cell number as a function of culture time and flow rate are shown.
As pointed out in the figure, statistically significant differences occur after 3, 5 and 7 culture days.
Cell number vs. flow rate
Click on image to enlarge