Bioreactors Perfusion Vascular Custom

Frequently Asked Questions

These are some questions frequently asked by other users of EBERS' products. If you have a new question, please post it to our support service.

Can... ?

Can I use the EBERS TEB1000 Master Unit as an ordinary incubator?

EBERS TEB1000 Master Unit maintains the basic capabilities of an ordinary incubator. The incorporation of the additional functionalities does no affect the original performance of the Unit as an incubator since constant 37ºC temperature and C02 concentration are guaranteed inside. However, using the Master Unit as an incubator might have a negative effect on pumps, engines and electronics used for their control, specially in 100% humidity conditions. Thus, we recommend to avoid creating high humidity conditions in the Unit in order to safeguard the electric machinery.

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Can your chambers be autoclaved?

Yes, all the chambers and accessories we supply are autoclavable at 121°C and 1.1 bar.

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Can I define and record my own flow profiles?

Yes. EBERS TEB1000 Master Unit allows defining and recording your customized flow profiles. For that purpose, three basic flow profiles are predefined in the bioreactor memory. By defining combinations of those profiles, you can easily create and store your flow profile so it is available for future cultures.

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Can the Master Unit be used with previously existing equipment?

Yes. Any equipment fitting inside the incubator - including tubing, accessories and culture chambers - may be used combined with the accessories provided by EBERS. None better than you can decide the equipment you need for your experiment.

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Can you transform my incubator into a flow bioreactor?

EBERS bioreactor is a product itself, not a transformed or rather adapted incubator. We do not transform your incubator into a flow bioreactor.

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Can you help me to develop my growth protocols?

EBERS bioreactors are general purpose and conceived to be used with many different kinds of tissue. Thus, the culturing conditions and needs for each type of culture might differ as a function of a multitude of factors, like cell and scaffold type or additive supply.

Thus, the experimental conditions growth conditions should be particularized as a function of many variables, which joined to the specified flow conditions, we determine the result of the experiment. Thus, developing a growth protocol requires of a deep knowledge of the experiment and the desired results, which will probably be far away from our understanding.

No one better than you can determine the most appropriate growing conditions for your particular experiment though, if possible, we would not hesitate in trying to advise you within our limits.

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What... ?

What is your service policy?

EBERS bioreactors are provided with a full two year warranty. All the pieces forming the bioreactors are built from subsystems that have been proven in thousands of applications.

In which the accessories is concerned, they warranty is directly provided by the manufacturer.

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What if I need assistance after-hours?

In case you need assistance after-hours, contact our technical service by telephone to get immediate contact with an EBERS engineer.

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What is needed to operate the bioreactor?

EBERS bioreactors are designed to be operated without using any additional equipment than the supplied by EBERS. Controls are fully integrated in the bioreactor, so there is no need of using a notebook to control the pumps functioning.

Control software is intuitive and easy to use without the need of reading a manual. The main testing parameters are placed at visible locations and are easy to set and modify. All of these features are easily controlled by means a tactile screen placed in the front of the bioreactor.

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How... ?

How can I increase the number of simultaneous experiments?

There are several ways in which you can easily increase the number of simultaneous experiments maintaining the same flow conditions. A relation of the most common strategies to increase the productivity of your experiments can be found here.

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How many samples can I grow simultaneously?

The number of samples you can grow simultaneously strongly depends on your experiment configuration and, in particular, of the culture chamber, the scaffold size the number of pumpheads you are using.

To give an example, we will assume that we are using two culture circuits - one in each pump - together with the EBERS perfusion package and 8 holes disks, which sums a total of 16 simultaneous samples. However, this number can be easily doubled just by using two pumpheads in each pump, which renders 32 sumultaneous samples. This examples gives an idea of the versatility of the Master Unit.

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