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Perfusion culture package
Culturing osseous and/or cartilaginous tissue on three-dimensional scaffolds in a bioreactor may be difficult due to the multiple scaffold sizes and textures available in the market, specially under perfusion loads.
EBERS offers a versatile chamber (patent pending) adaptable to be used with scaffolds of different sizes and consistency. It consists of two cylindrical conically holed parts designed to eliminate the turbulences occurring in the fluid stream and two discs that host the scaffolds inside.
The main parts of this culture package are described below.
Main Body
This chamber has been created for the culture of scaffolds under perfusion loads and, specifically, to the growth of bone and cartilage scaffolds with a good consistency and resistance to the forces due to the culture media flow.
The chamber is composed of two holed parts, which are conically-shaped in order to channel the flow to the samples minimising the flow turbulence.
Scaffolds are placed in between both parts of the body chamber to carry out the culture. Then, the chamber is hermetically locked in order to prevent media leakage and culture contamination. Watertightness is assured by means of closure gasket.
Once the chamber is locked, the system is ready to be connected to the culture circuit. The number of tube docks may be variable depending on the particular needs of the experiment.
A disc in between those two parts serves to hold the scaffolds in which cells will be grown. That disc is configured ad-hoc for you scaffold dimensions, and can be easily interchanged if required.
Interchangeable disks sets
Using interchangeable disks sets to place the scaffold is the main advantage of this chamber.
Disks are also made of the same material than the main body of the chamber. Each set of disk is composed of two drilled disks in which the scaffold (or scaffolds) are placed.
The diameter and shape of the drills is usually custom-made to fit the dimension of each particular scaffold. Since the disks are interchangeable, it is possible to used the same chamber to grow very different scaffolds sizes.
Furthermore, increasing the number of simultaneous tests under the same culture conditions is also possible by drilling several holed in the same disks set. Thereby, the productivity of the experiments is increased in a simple and efficient way.
Visit the technical specifications page for further information on the EBERS perfusion chambers.