The Bio-Byblos Cellusponge are three-dimensional porous hydroxipropylcellulose (HPC) scaffolds which have been designed to be used with cells not requiring specific ligands. Moreover than the standard non-coated cellusponge, the same type of scaffold is available with two different coatings to better adapt to different cell types. Cellusponge is supplied in cylindindrical shapes of 6 and 9 mm in diameter and 1 mm in height with a well-controlled pore size between 100 and 200 microns.
Depending on the desired coating, the Cellusponge scaffolds show an optimal performance for the development of a particular type of experiment.
It is intended for the development of general soft tissue 3D culture. Cell types that have been successfully used with this scaffolds are MCF-7 and MDA-MDB, mouse fibroblasts NIH3T3, human foreskin fibroblast HFF.
Cellusponge has been used as soft matrix for 3D cell culture and 3D tumour model.
It is designed for primary rat and human cryopreserved hepatocytes and various hepatocyte cell lines HepG2,C3A and Huh7.5. Moreover, it can be applied in in-vitro drug safety testing, 3D cell culture model for hepatitis virus infection and replication study, and hepatocyte-like cells maturation in differentiation study.
It has been successfully used in stem cells differentiation experiments. Mouse and human embryonic stem cells (mESC and hESC), human mesenchymal stem cells (hMSC) and cardiomyocytes have been sucessfully cultured in this type of scaffold.
Cellusponge scaffolds are supplied in 6 and 9 mm diameter disks with a height of 1 mm in different packages. Contact us to know more about the available packages and to receive a quotation or visit the Bio-Byblos site for further information.
What is 3D CelluSponge made of?
A: It is made of hydroxypropyl cellulose, a known biocompatible material, as the basic material which was side-chain modified for crosslinking purpose and ligand conjugation.
Is 3D CelluSponge easy to use?
A: Yes, the cell seeding is as easy as conventional 2D culture.
How do I seed cells onto 3D CelluSponge?
A: The cell seeding can be easily performed through absorption of cell suspension onto the surface of dry sponge. Firstly the cell suspension of desired cell density is dropped onto the dry sponge surface until the sponge absorbs the liquid completely. After 30-60 minutes of incubation, fresh pre-warmed culture medium can be added for extended cell culture maintenance. For further information, please kindly contact us and we will be pleased to advise you.
Does 3D CelluSponge come ready to use?
A: Yes, the sponge comes in your desired formats, pre-sterilized and ready for cell seeding experiments.
Are there any published papers utilizing this sponge?
A: Yes, there are some papers such as Yue et al. Biomaterials 2010, Gu et al. Regenerative Medicine 2010 & Nugraha et al. Biomaterials 2011.
Does 3D CelluSponge come with well insert and or single unit?
A: Yes, it comes in both formats. However we can also customize any formats based on your request and needs.
Can the 3D cellusponge be re-used?
A: We do not recommend re-using the 3D cellusponge. The 3D cellusponges are designed for single-use experiments.
What cell types can I grow into spheroids using the 3D cellusponge?
A: Our users have successfully demonstrated spheroid formation using many cell types.
Why aren’t my cells forming spheroids?
A: Not all cell types form spheroids readily when cultured in 3D cellusponge. Please check relevant literature to see if spheroid formation with your cell type of interest has been demonstrated before. Media composition is an important factor to spheroid formation, so make sure your culture media contains the necessary supplements.
How long does spheroid formation take?
A: Spheroid formation requires about 3-7days once inoculated. Air bubbles will be observed at time of inoculation. This will disappear with several days by the cells consuming the oxygen.
Usually users grow cells for 3 to 4 days and at certain density, they subculture cells. How can they subculture in this sponge?
A: Spheroids can be dissociated with Trypsin-EDTA (or TrypLE), counted and expanded into other culture vessels or into new sponges.